Tuesday, 28 February 2012

Haemocytometer: Determining Cell concentration

1.       20μl of media containing cells was taken in an eppendorf to which 20μl of trypan blue was added and mixed well
2.       The cover slip was placed on the haemocytometer and 10μl of this solution was loaded to one side of the clean haemocytometer and 10μl more was loaded on to the other side and allowed it to fill by capillary action.
3.       4×4 = 16 blocks on one corner was found and cells were counted with 10X objective. Cells were not counted if they touch the right hand or bottom borders of the square.
4.       The cell number obtained was calculated in a ml
a.       Average of the four squares was calculated
b.      This was multiplied by 104

Extra Information:
·         Haemocytometer was cleaned with ethanol before use.
·         Dead cells take up trypan blue but not viable cells.
·         Each large square measure 1mm x 1mm, 0.1mm deep.
·         Each square represents a volume of 0.1mm3 (1.0mm2area x 0.1mm deep) or    10-4cm3
·         Cell number is usually expressed in cm3 and is determined by multiplying the average of the no. of cells counted by a conversion factor which is constant for the haemocytometer. The conversion factor is estimated at 1000, based on the fact each large square represents a total volume of 10-4cm3
Total cells =(no.of cells counted)/(no.of large squares counted) x conversion factor x dilution factor x total volume of suspension

Tuesday, 6 December 2011

Grammar for the day -12/7 2011


Unique is an adjective that means 'one of a kind' and it cannot be graduated.

Therefore, it is incorrect to say 'more unique' or 'very unique'.
Such forms are used in informal conversations, but are frowned upon in formal speech.

Monday, 6 June 2011

Prepare 0.01% poloxamer in acetone solution

In order to prepare 10ml of 0.01% poloxamer 188 in acetone, you need to add 1mg of poloxamer 188 in 10 ml of acetone.
Assuming 1% = 1g/100ml
0.01% = 0.01g/100ml
0.01% = 10mg/100ml
0.01% = 1mg/10ml

Tuesday, 17 May 2011

Scientific jobs UK

Biomedical, Biotechnology, Life Science, Scientific, Pharmaceutical Job sites (Consultants) UK
Here is a list of various job sites that posts various Life science (Biotechnology, Biomedical, pharmaceutical, clinical, health) jobs. Almost all websites allows you to register and apply jobs online.

1. Nature jobs

2. Jobs.ac.uk

3. Reed

4. Camco

5. CK science

6. Jobsite

7. SRG

8. Total jobs

9. Matchtech

10. Access-science

11. Cranleigh scientific

12. Pennywarren

13. Sci-search

14. Hudson-shribman

15. New Scientist jobs

16. Kinetica

17. Seltek

18. Lab support

19. Pulse

20. Jobs word

21. 1Job

22. CV library

23. Kelly services

24. Science-techrecruitment

Monday, 18 August 2008

phd vacancies and other jobs in life science

The best jobs in all life science related fields are advertised in the website of 'Nature'. It brings the vacancies from around the globe, particularly from Europe and America